| 货号 | 5263S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Oct-4A (C30A3) Rabbit mAb #2840. |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that it is the Oct-4A isoform that has the ability to confer and sustain pluripotency and that Oct-4B may exist in some somatic, non-pluripotent cells (6,7). |
| 存放说明 | 4C |
Flow cytometric analysis of HeLa cells (blue) and NTERA-2 cells (green) using Oct-4A (C30A3) Rabbit mAb (Alexa Fluor® 647 Conjugate). 使用Oct-4A (C30A3) Rabbit mAb兔单抗(Alexa Fluor® 647 偶联)对HeLa细胞（蓝色）和NTERA-2细胞（绿色）进行流式细胞分析。 | |
Confocal immunofluorescent analysis of NTERA-2 (left) and HeLa cells (right) using Oct-4A (C30A3) Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments have been labeled with DY-554 phalloidin (red). 使用Oct-4A (C30A3) Rabbit mAb兔单抗(Alexa Fluor® 647 偶联) (蓝色伪彩)对NTERA-2（左）和HeLa细胞（右）进行激光共聚焦荧光分析。 |
