p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Alexa Fluor® 488 Conjugate)
货号：
4780S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 4780S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Mink/D.melanogaster/Zebrafish/Bovine/Dog/Pig/C.elegans |
| 来源宿主 | Rabbit |
| 应用 | F |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059. |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of Jurkat cells, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) compared to XP® Rabbit (DA1E) mAb IgG Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (blue).Flow cytometric方法检测细胞提取物：Jurkat 细胞，使用的抗体是p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Alexa Fluor® 488 Conjugate)(绿色)，蓝色曲线表示使用的抗体是XP® Rabbit (DA1E) mAb IgG Isotype Control (Alexa Fluor® 488 Conjugate) #2975。 |
