| 货号 | 4279S |
| 描述 | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated Pan-Keratin (C11) Mouse mAb #4545 reacts with human, rat and monkey pan-keratin. CST expects that Pan-Keratin (C11) Mouse mAb (Biotinylated) will also recognize pan-keratin in these species. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/F |
| 目标/特异性 | Pan-Keratin (C11) Mouse mAb (Biotinylated) detects endogenous levels of total keratin 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins. |
| 使用方法 | WB(1:1000) F (1:100) |
| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6). |
| 存放说明 | -20C |
| 计算分子量 | 46-58 |
Western blot analysis of extracts from HeLa and H-4-II-E cells using Pan-Keratin (C11) Mouse mAb (Biotinylated) detected using Streptavidin-HRP #3999.使用Pan-Keratin (C11) Mouse mAb (Biotinylated)，其检测使用Streptavidin-HRP #3999，免疫印迹（Western Blot）分析HeLa和H-4-II-E细胞中Pan-Keratin蛋白水平。 | |
Flow cytometric analysis of MCF7 cells using Pan-Keratin (C11) Mouse mAb (Biotinylated) (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) #4097 (red).与Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) #4097 (红色)比较，使用Pan-Keratin (C11) Mouse mAb (Biotinylated) (蓝色)标记，流式细胞仪分析MCF7细胞。 |
