| 货号 | 8580S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated PCNA (PC10) Mouse mAb #2586.该抗体偶联了Alexa Fluor® 488 荧光染料，并且使用人类细胞采用直接流式细胞术和免疫荧光方法通过了内部测试。该抗体预计与未标记PCNA (PC10) Mouse mAb #2586有相同的种属交叉反应性。 |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine/Pig |
| 来源宿主 | Mouse |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:400) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies.增殖细胞核抗原(PCNA)是DNA滑动压板蛋白家族的一员，该蛋白家族能够协助DNA复制(1)。晶体结构数据表明一个PCNA同源二聚体环能够沿着DNA双螺旋包绕和滑动(2)。多种蛋白参与了DNA复制和DNA修复，细胞周期控制结合PCNA而非直接联合DNA，从而促进了DNA的快速处理(3中已论述)。PCNA蛋白表达是一个公认的细胞增殖标志，而且PCNA (PC10)小鼠单抗已被用来评估众多研究中PCNA的水平。 |
| 存放说明 | 4C |
Confocal immunofluorescent analysis of NCI-H460 cells using PCNA (PC10) Mouse mAb (Alexa Fluor® 488 Conjugate) (green), p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 647 Conjugate) #8587 (blue pseudocolor), and Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #3475 (red).激光共聚焦免疫荧光方法检测 NCI-H460 细胞，使用的抗体为PCNA (PC10) Mouse mAb (Alexa Fluor® 488 Conjugate)，呈绿色，p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 647 Conjugate) #8587 ，为蓝色伪彩 ， Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #3475，呈红色。 | |
Flow cytometric analysis of Jurkat cells using PCNA (PC10) Mouse mAb (Alexa Fluor® 488 Conjugate) and propidium iodide (DNA content).流式细胞术检测Jurkat细胞，使用的抗体为PCNA (PC10) Mouse mAb (Alexa Fluor® 488 Conjugate) ，碘化乙啶检测DNA含量作为对照。 |
