Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (PE Conjugate)
货号：
7547S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 7547S |
| 描述 | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855. |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (PE Conjugate) detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | PE |
| 背景 | Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue), using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (PE Conjugate). 流式细胞术分别检测未处理（绿色）或经LY294002 #9901, Wortmannin #9951, 以及U0126 #9903处理（蓝色）的Jurkat细胞活性，使用的抗体为Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb兔单抗 (PE接合)。 |
