| 货号 | 3929S |
| 描述 | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855. |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at analagous sites. |
| 使用方法 | WB(1:1000) F (1:400) |
| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). |
| 存放说明 | -20C |
| 计算分子量 | 15 to 20 |
Western blot analysis of extracts from HeLa cells, serum-starved and stimulated with insulin (100 nM for 5 minutes), with (+) or without (-) λ phosphatase treatment, using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated) (upper) and 4E-BP1 (53H11) Rabbit mAb #9644 (lower). 使用Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated) (上图)和4E-BP1 (53H11) Rabbit mAb兔单抗 #9644 (下图)，Western Blot分析在HeLa细胞系中磷酸化4E-BP1和4E-BP1蛋白的水平。细胞在无血清饥饿培养后再用胰岛素刺激(100 nM，5分钟)，然后分为λ磷酸酶处理组（+）和未处理组。 | |
Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002, Wortmannin and U0126-treated (blue), using Phospho-4E-BP1 (Thr36/46) (236B4) Rabbit mAb (Biotinylated). 使用Phospho-4E-BP1 (Thr36/46) (236B4) Rabbit mAb兔单抗 (Biotinylated)，流式细胞术分析Jurkat细胞，细胞分为未处理组（绿色）和LY294002, Wortmannin 和U0126处理组（蓝色）。 |
