Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate)
货号：
11865S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 11865S |
| 描述 | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in monkey cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Bad (Ser112) (40A9) Rabbit mAb #5284. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate) detects endogenous levels of Bad only when phosphorylated at Ser112. The Ser112 nomenclature is based upon the mouse sequence. The analogous phosphorylation site is Ser75 in human and Ser113 in rat. This antibody does not detect Bad phosphorylated at other sites, nor does it detect related family members. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | PE |
| 背景 | Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of COS-7 cells, treated with Staurosporine #9953 (blue) or TPA #4174 (green), using Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate). 流式细胞术分析COS-7细胞，Staurosporine #9953（蓝色）或 TPA #4174（绿色）处理，使用抗体为 Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate)兔单抗。 |
