| 货号 | 9187S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human and mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198. |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | IF-F/IF-IC/F |
| 目标/特异性 | Phospho-CREB (Ser133) (87G3) (Alexa Fluor® 488 Conjugate) Rabbit mAb detects endogenous levels of CREB only when phosphorylated at serine 133. The antibody also detects the phosphorylated form of the CREB-related protein, ATF-1. |
| 使用方法 | F(1:50) IF-F (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9). |
| 存放说明 | 4C |
Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or PDGF treated (green), using Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor® 488 Conjugate) (#9187). 流式细胞仪分析NIH/3T3细胞，未经处理（蓝色）或经过PDGF处理（绿色），使用Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor® 488 Conjugate) (#9187)进行标记。 | |
Confocal immunofluorescent analysis of P14 rat cerebellum, untreated (left) or phosphatase treated (right), labeled with Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 共聚焦免疫荧光分析P14大鼠小脑，未经处理（左图）或经过磷酸化处理（右图），使用Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor® 488 Conjugate)（绿色）和GFAP (GA5) Mouse mAb 鼠单抗 #3670（红色）进行标记。蓝色伪彩=DRAQ5™(荧光DNA染料)。 | |
Confocal immunofluorescent analysis of SK-N-MC cells, treated with Forskolin and IBMX (left) or untreated (right), labeled with Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 共聚焦免疫荧光分析SK-N-MC细胞，经Forskolin和IBMX处理（左图）或未经处理（右图），使用Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor® 488 Conjugate)（绿色）进行标记。肌动蛋白纤丝用Alexa Fluor® 555 phalloidin（红色）标记。 |
