Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor® 647 Conjugate)
货号：
4887S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 4887S |
| 描述 | Cell Signaling Technology Antibody conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct Flow Cytometric analysis of human cells. The unconjugated antibody, #3033, reacts with Phospho-NF-KappaB p65 (Ser536) from human, mouse. and rat. CST expects that Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor® 647 Conjugate) will also recognize Phospho-NF-KappaB p65 (Ser536) in these species. |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Pig |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects NF-kappaB p65 only when phosphorylated at serine 536. It does not cross-react with the p50 subunit or other related proteins. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of THP-1 cells, untreated (blue) or TNF-α treated (green), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor® 647 Conjugate).流式细胞仪研究未经处理的THP-1细胞(蓝色)或经TNF-α处理的THP-1细胞(绿色)。所用抗体为Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor® 647 Conjugate)。 |
