Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate)
货号：
5733S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 5733S |
| 描述 | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033. |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Pig |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (PE conjugate) detects NF-κB p65 only when phosphorylated at Ser536. It does not cross-react with the p50 subunit or other related proteins. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | PE |
| 背景 | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of HeLa cells, untreated (blue) or treated with TNF-α #8902 and Calyculin A #9902 (green), using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate). |
