Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb (PE Conjugate)
货号：
12428S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 12428S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (PE Conjugate) recognizes endogenous levels of Smad1 protein when phosphorylated at Ser465 only, at both Ser463 and Ser465, or at Ser462, Ser463 and Ser465. This antibody also recognizes the endogenous levels of Smad5 protein when phosphorylated at Ser463 only, Ser465 only, at both Ser463 and Ser465, at both Ser462 and Ser465, or at Ser462, Ser463 and Ser465. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | PE |
| 背景 | Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of HT-1080 cells, untreated (blue) or treated with Human BMP2 #4697 (50 ng/ml, 30 min; green), using Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb (PE Conjugate). 未处理(蓝色)或经过Human BMP2 #4697 (50 ng/ml, 30 min; 绿色)处理的HT-1080细胞，使用Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit mAb兔单抗 (PE 偶联)进行流式细胞分析。 |
