Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (PE Conjugate)
货号：
8062S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 8062S |
| 描述 | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167. |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (PE Conjugate) recognizes endogenous levels of Stat1 only when phosphorylated at Tyr701. The antibody detects phosphorylated Tyr701 of both Stat1α and Stat1β. It does not cross-react with the corresponding phospho-tyrosines of other Stat proteins. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | PE |
| 背景 | The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with hIFN-α1 #8927 (green), using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (PE Conjugate).流式细胞仪研究未经处理的Jurkat细胞(蓝色)和经IFN-α#8927 处理的Jurkat细胞(绿色)。所用抗体为Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (PE Conjugate)。 |
