Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 488 Conjugate)
货号：
9414S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 9414S |
| 描述 | Cell Signaling Technology Antibody conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells.
*The unconjugated antibody, #9411, reacts with phospho-tyrosine in all species tested. CST expects that Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 488 conjugate) will also recognize phospho-tyrosine in all species.CST公司生产的抗体在最优条件下与Alexa Fluor® 488荧光染料结合，已通过在人类细胞上进行直接流式细胞反应的内部测试。*未结合抗体#9411与所有种属的磷酸化酪氨酸反应。CST预计Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 488 conjugate)也可以识别所有物种的磷酸化酪氨酸。 |
| 反应种属 | Human/Mouse/Rat/All |
| 来源宿主 | Mouse |
| 应用 | F |
| 目标/特异性 | Phospho-Tyrosine Mouse mAb (P-Tyr-100) is a high affinity antibody. ELISAs against a wide variety of phosphopeptides indicate that (a) P-Tyr-100 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel Western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-100 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-100 does not cross-react with peptides containing phospho-Ser or phospho-Thr. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of K562 cells, untreated (green) or Gleevec®- treated (blue), using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 488 Conjugate) compared with a nonspecific negative control antibody (red). 对K562细胞，未处理（绿）或Gleevec®处理（蓝），使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)(Alexa Fluor® 488 Conjugate)和非特异性阴性对照抗体（红色），进行流式细胞仪分析。 |
