PU.1 (9G7) Rabbit mAb (Alexa Fluor® 488 Conjugate)
货号：
2216S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 2216S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. The unconjugated antibody #2258 reacts with human and mouse PU.1 protein. CST expects that PU.1 (9G7) Rabbit mAb (Alexa Fluor® 488 Conjugate) will also recognize PU.1 in these species. |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | PU.1 (9G7) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total PU.1 protein. This antibody does not cross react with other Ets family members. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | PU.1 is a member of the Ets family of transcription factors and activates target genes through the purine-rich PU-box (1). PU.1 plays a pivotal role in the differentiation of myeloid cells and lymphocytes and is expressed in several hematopoietic cells including B lymphocytes, macrophages, neutrophils, mast cells, early erythroid cells and megakaryocytes (1,2). The concentration of PU.1 is critical for both the determination of hematopoietic cell lineage and the regulation of differentiation versus stem cell proliferation (3,4). In addition, PU.1 activity is influenced by phosphorylation and interactions with other hematopoietic transcription factors. Phosphorylation of PU.1 at Ser146 by CK2 promotes binding to IRF4 and synergistic activation through the immunoglobulin κ 3 enhancer (5). Treatment of pro-B cells with IL-3 leads to phosphorylation of PU.1 at Ser140, resulting in increased PU.1 activity and activation of the anti-apoptotic gene MCL-1 (6). GATA1 binding blocks PU.1 activity during erythroid cell development (7). Overexpression of PU.1 resulting from proviral insertion during Friend virus infection can induce erythroleukemia, while reduced expression has been associated with acute myeloid leukemia (8). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of MCF-7 cells (blue) or THP-1 cells (green) using PU.1 (9G7) Rabbit mAb (Alexa Fluor® 488 Conjugate).流式细胞仪分析MCF-7细胞(蓝色) 和THP-1(绿色)细胞。所用抗体为PU.1 (9G7) Rabbit mAb (Alexa Fluor® 488 Conjugate)。 |
