S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 488 Conjugate)【科瑞奥博】

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S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 488 Conjugate)

货号： 5317S 基本售价： 4060.0 元规格： 100ul

产品信息

概述
货号	5317S
描述	This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated S6 Ribosomal Protein (54D2) Mouse mAb #2317.
反应种属	Human/Mouse/Rat/Monkey/D.melanogaster
来源宿主	Mouse
应用	IF-F/IF-IC/F
目标/特异性	S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total S6 ribosomal protein independent of phosphorylation.
使用方法	F(1:50) IF-F (1:400) IF-IC (1:400)

性能
供应商	CST
标记	Alexa Fluor 488
背景	One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
存放说明	4C

参考图片

Flow cytometric analysis of Jurkat cells, using S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 488 Conjugate) (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (red).

与Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (红色)比较，使用S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 488 Conjugate) (蓝色)标记，流式细胞仪分析Jurkat细胞。

Confocal immunofluorescent analysis of HeLa cells using S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

使用S6 Ribosomal Protein (54D2) (Alexa Fluor® 488) Mouse mAb鼠单抗 (绿色)标记，共聚焦免疫荧光观察S6 Ribosomal Protein蛋白在HeLa细胞中定位。DY-554 phalloidin标记微丝蛋白（红色）。蓝色伪彩= DRAQ5® (DNA荧光染料)。