| 货号 | 5548S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated S6 Ribosomal Protein (54D2) Mouse mAb #2317. |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster |
| 来源宿主 | Mouse |
| 应用 | IF-F/IF-IC/F |
| 目标/特异性 | S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total S6 ribosomal protein. |
| 使用方法 | F(1:50) IF-F (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).生长因子和有丝分裂原有效促进维持细胞生长和增殖的一个方面就是通过上调mRNA翻译(1,2)。生长因子和有丝分裂原能诱导p70 S6激酶的激活和随后S6核糖体蛋白的磷酸化。S6核糖体蛋白的磷酸化与mRNA转录本的翻译增加有关，而这个mRNA转录本是在5端非翻译区含有一个寡嘧啶区(2)。这些特殊的mRNA转录本 (5TOP) 能够编码涉及细胞周期进程的蛋白质如翻译必须的核糖体蛋白和延伸因子(2,3)。重要的S6核糖体蛋白的磷酸化位点包含数个定位在S6蛋白一个小的羧基末端区域残基(Ser235, Ser236, Ser240, and Ser244) (4,5)。 |
| 存放说明 | 4C |
Flow cytometric analysis of Jurkat cells, using S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) (green) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 647 Conjugate) #4843 (red). 与Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 647 Conjugate) #4843 (红色)比较，使用S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate)标记，流式细胞仪分析Jurkat细胞。 | |
Confocal immunofluorescent analysis of HeLa cells using S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor), β-Catenin (L54E2) Mouse mAb (IF Preferred) (Alexa Fluor® 555 Conjugate) #5612 (red) and Histone H3 Antibody #9715 (green). 使用S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) (蓝色) ，β-Catenin (L54E2) Mouse mAb (IF Preferred) (Alexa Fluor® 555 Conjugate) #5612 (红色)和Histone H3 Antibody #9715 (绿色)标记，共聚焦免疫荧光观察S6 Ribosomal Protein蛋白在HeLa细胞中定位。 | |
Confocal immunofluorescent analysis of rat brain using S6 Ribosomal Protein (54D2) (Alexa Fluor® 647) Mouse mAb (blue pseudocolor) and β3-Tubulin (D71G9) XP® Rabbit mAb #5568 (green). Red = Propidium Iodide (fluorescent DNA dye). 使用S6 Ribosomal Protein (54D2) (Alexa Fluor® 647) Mouse mAb (蓝色) ，β3-Tubulin (D71G9) XP® Rabbit mAb #5568 (绿色)标记，共聚焦免疫荧光观察S6 Ribosomal Protein蛋白在大鼠大脑中定位。红色=碘化丙啶(DNA荧光染料)。 |
