| 货号 | 14047S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:100) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 存放说明 | 4C |
Flow cytometic analysis of PC-3 (blue) and HeLa (green) cells using Stat3 (D3Z2G) Rabbit mAb (Alexa Fluor® 488 Conjugate). | |
Confocal immunofluorescent analysis of HeLa cells (serum-starved overnight), untreated (upper left) or treated with Human Interferon-α1 (hIFN-α1) (1,000 units/ml, 30 min; upper right), and PC-3 cells (serum-starved overnight), untreated (lower left) or treated with Human Interferon-α1 (hIFN-α1) (1,000 units/ml, 30 min; lower right), using Stat3 (D3Z2G) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). |
