| 货号 | 2810S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Survivin (71G4B7) Rabbit mAb #2808. |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6). |
| 存放说明 | 4C |
Flow cytometric analysis of untreated (green) and etoposide treated (blue) Jurkat cells, using Survivin (71G4) (Alexa Fluor® 488 conjugate) Rabbit mAb showing a decrease in survivin expression in apoptotic cells. 流式细胞仪检测Jurkat细胞，未处理（绿）和 etoposide 处理（蓝），使用抗体为Survivin (71G4) (Alexa Fluor® 488 conjugate) Rabbit mAb 兔单抗，显示了凋亡细胞中survivin表达的下降。 | |
Confocal immunofluorescent image of HeLa cells labeled with Survivin (71G4) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光成像HeLa细胞，使用抗体为Survivin (71G4) Rabbit mAb (Alexa Fluor® 488 Conjugate)兔单抗（绿色）。Alexa Fluor® 555 phalloidin 标记肌动蛋白丝（红色）。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 |
