| 货号 | 5875S |
| 描述 | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Survivin (71G4B7) Rabbit mAb #2808. |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Survivin (71G4B7) Rabbit mAb (PE Conjugate) detects endogenous levels of total Survivin protein. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 标记 | PE |
| 背景 | Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4).Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6). |
| 存放说明 | 4C |
Flow cytometric analysis of Jurkat cells using Survivin (71G4B7) Rabbit mAb (PE Conjugate) (green) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (red). 流式细胞术检测Jurkat细胞，使用抗体为Survivin (71G4B7) Rabbit mAb (PE Conjugate)兔单抗（绿色），一个浓度相匹配的 Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 （红色）作为对照。 | |
Flow cytometric analysis of camptothecin-treated Jurkat cells using Survivin (71G4B7) Rabbit mAb (PE Conjugate) showing a decrease in survivin expression in apoptotic cells (blue) compared to non-apoptotic cells (green). 流式细胞仪检测 camptothecin 处理的Jurkat细胞，使用抗体为Survivin (71G4B7) Rabbit mAb (PE Conjugate)兔单抗，显示了凋亡细胞中survivin表达的下降（蓝色），对照是一个非凋亡细胞（绿色）。 |
