| 货号 | 77143S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | IRAK1 (D51G7) Rabbit mAb (PE Conjugate) detects endogenous levels of total IRAK1 protein. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3). |
| 存放说明 | 4C |
| 参考文献 | 1 . Dinarello, C.A. (1996) Blood 87, 2095-147. 2 . Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84. 3 . Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302. |
Flow cytometric analysis of 293T cells untreated (green) or transfected with CRISPR/IRAK1 KO plasmid (blue) using IRAK1 (D51G7) Rabbit mAb (PE Conjugate). |
