| 货号 | 13871S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | c-Myc (D84C12) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total c-Myc protein. This antibody is not recommended for detection of Myc-tagged fusion proteins; for detection of Myc-tagged fusion proteins, use Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) #2233. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 标记 | Alexa Fluor 647 |
| 背景 | Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4). |
| 存放说明 | 4C |
| 参考文献 | 1 . Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702. 2 . Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7. 3 . Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82. 4 . Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99. |
Flow cytometric analysis of Jurkat cells using c-Myc (D84C12) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (green) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (red). |
