| 货号 | 5251S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | Phospho-TCTP (Ser46) Antibody recognizes endogenous levels TCTP protein only when phosphorylated at Ser46. In some cell types the antibody may recognize an 80-100 kDa protein of unknown origin. |
| 使用方法 | WB(1:1000) IHC-P (1:100) F (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Translationally controlled tumor protein (TCTP/p23/HRF) is a ubiquitously expressed and highly conserved protein involved in various cellular processes, including a role as a histamine releasing factor in chronic allergic disease (1). TCTP binds tubulin in a cell cycle dependent manner and is associated with the mitotic spindle (2). In addition, TCTP interacts with the actin cytoskeleton to regulate cell shape (3). In mitosis, TCTP is phosphorylated by PLK at Ser46, decreasing microtubule stability (4,5). TCTP interacts with the small GTPase Rheb, possibly acting as a GEF and thus activating the TORC1 pathway, controlling cell growth and proliferation (6,7). TCTP has also been shown to be involved in apoptosis and cell stress (8-11). In cultured cells, reduction of TCTP expression can cause tumor reversion (12). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
Western blot analysis of extracts from U-2 OS cells, untreated or synchronized in mitosis by thymidine block followed by release into nocodazole, using Phospho-TCTP (Ser46) Antibody. 使用Phospho-TCTP (Ser46) Antibody，免疫印迹（Western Blot）分析U-2 OS细胞中Phospho-TCTP蛋白水平。细胞分为untreated或Mitotic synchrony通过使用thymidine封闭随后nocodazole处理。 | |
Confocal immunofluorescent analysis of U-2 OS cells using Phospho-TCTP1 (Ser46) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Phospho-TCTP1 (Ser46) Antibody (绿色)标记，共聚焦免疫荧光分析U-2 OS细胞。DY-554 phalloidin标记微丝蛋白(红色)。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。 | |
Flow cytometric analysis of Jurkat cells using Phospho-TCTP (Ser46) Antibody compared to propidium iodide (DNA content). 与propidium iodide (DNA含量)比较，使用Phospho-TCTP (Ser46) Antibody，流式细胞仪分析Jurkat细胞。 | |
Immunohistochemical analysis of paraffin-embedded HT-29 cell pellets, control (left) or nocodazole-treated (right), using Phospho-TCTP (Ser46) Antibody. 使用Phospho-TCTP (Ser46) Antibody，免疫组化分析HT-29细胞石蜡切片，组织分为control (左图)或nocodazole-treated (右图)。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or lambda-phosphatase-treated (right), using Phospho-TCTP (Ser46) Antibody. 使用Phospho-TCTP (Ser46) Antibody，免疫组化分析人源乳腺癌组织石蜡切片，组织分为control (左图)或lambda-phosphatase-treated (右图)。 |
