| 货号 | 2211S |
| 反应种属 | Human/Mouse/Rat/Monkey/S.cerevisiae |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IHC-F/IF-IC/F |
| 目标/特异性 | Phospho-S6 Ribosomal Protein (Ser235/236) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 and 236. This antibody does not detect ribosomal protein S6 phosphorylated at other sites. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:400) F (1:100) IF-IC (1:100) IHC-F (1:200) |
| 供应商 | CST |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
Western blot analysis of extracts from 293 cells, untreated or treated with 20% FBS for the indiccated time, using , Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (upper) or Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 (lower). 使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (上图)和Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 (下图)，免疫印迹（Western blot）分析293细胞，分为对照组和20% FBS处理组（如图中所示的时间）。 | |
Immunohistochemical staining of paraffin-embedded human melanoma, showing cytoplasmic localization of phosphorylated S6 ribosomal protein, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. 使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody，免疫组化分析人类黑色素瘤组织石蜡切片，结果显示磷酸化的S6核糖体蛋白定位在细胞质。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody preincubated with control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (right). 使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody，免疫组化分析人类乳腺癌组织石蜡切片，其分别孵育对照多肽(左图)和Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (右图)。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or calf-intestinal phosphatase (CIP) treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. 使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody，免疫组化分析人类乳腺癌组织石蜡切片，分为对照组（左图）和牛小肠磷酸酶(CIP)处理组（右图）。 | |
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. | |
使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody，免疫组化分析LNCaP细胞石蜡切片，分为对照组（左图）和rapamycin处理组（右图）。 | |
Imunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. 使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody，免疫组化分析卵巢癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. 使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody，免疫组化分析人类肾脏细胞癌石蜡切片。 | |
Flow cytometric analysis of NIH/3T3 cells, U0126- and Rapamycin-treated (blue) or serum-treated (green), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody compared to a nonspecific negative control antibody (red). 与非特异阴性对照抗体(红色)，使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody标记，流式细胞术分析NIH/3T3细胞，细胞分为U0126和Rapamycin处理组(蓝色)和血清处理组(绿色)。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. 使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody，免疫组化分析人类肺癌组织石蜡切片。 |
