| 货号 | 2406S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IHC-F/IF-IC/F |
| 目标/特异性 | Phospho-HSP27 (Ser82) Antibody II detects endogenous HSP27 only when phosphorylated at Ser82. The antibody does not recognize other heat shock proteins. |
| 使用方法 | WB(1:1000) IHC-P (1:100) F (1:50) IF-IC (1:400) IHC-F (1:100) |
| 供应商 | CST |
| 背景 | Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78 and Ser82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 27 |
Flow cytometric analysis of HeLa cells, untreated (blue) or UV-treated (green), using Phospho-HSP27 (Ser82) Antibody II. 对未处理（蓝）或紫外线处理（绿）的Hela细胞使用Phospho-HSP27 (Ser82) Antibody II进行流式细胞术分析。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using Phospho-HSP27 (Ser82) Antibody II (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对HeLa细胞，未处理（左）或anisomycin处理（右），使用Phospho-HSP27 (Ser82) Antibody II（绿色）进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor®555 phalloidin（红色）标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-HSP27 (Ser82) Antibody II. 对石蜡包埋的人肺癌使用Phospho-HSP27 (Ser82) Antibody II进行免疫组化分析。 | |
Immunohistochemical analysis of frozen H1650 xenograft using Phospho-HSP27 (Ser82) Antibody II. 对冰冻的H1650异种移植物使用Phospho-HSP27 (Ser82) Antibody II进行免疫组化分析。 | |
Western blot analysis of extracts from HeLa and PC12 cells, treated with λ-phosphatase or UV as indicated, using Phospho-HSP27 (Ser82) Antibody II. 对HeLa和PC12细胞，按要求进行λ磷酸酶或紫外线处理，使用Phospho-HSP27 (Ser82) Antibody II进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human melanoma using Phospho-HSP27 (Ser82) Antibody II. 对石蜡包埋的人黑色素瘤使用Phospho-HSP27 (Ser82) Antibody II进行免疫组化分析。 |
