| 货号 | 3181S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/F |
| 目标/特异性 | Phospho-CrkL (Tyr207) Antibody detects endogenous levels of CrkL only when phosphorylated at tyrosine 207. The antibody cross-reacts with CrkII phosphorylated at tyrosine 221. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) F (1:50) |
| 供应商 | CST |
| 背景 | CrkL, a 39 kDa adaptor protein, has a key regulatory role in hematopoietic cells. CrkL has one SH2 and two SH3 domains, with 60% homology to CrkII (1). The amino-terminal SH3 domain of CrkL binds proteins such as C3G, SOS, PI3K, c-Abl and BCR/Abl. The SH2 domain of CrkL can bind to tyrosine-phosphorylated proteins such as Cbl, HEF1, CAS and paxillin (2,3). CrkL is involved in various signaling cascades initiated by different cytokines and growth factors. The biological outcomes of the Crk-activated signal transduction include the modulation of cell adhesion, cell migration and immune cell responses (4). CrkL is a prominent substrate of the BCR/Abl oncoprotein in chronic myelogenous leukemia and binds to both BCR/Abl and c-Abl (5). CrkL is prominently and constitutively tyrosine phosphorylated in CML neutrophils and is not phosphorylated in normal neutrophils. Moreover, stimulation of normal neutrophils with cytokines and agonists does not induce tyrosine phosphorylation of this protein (6), indicating that it may be a useful target for therapeutic intervention or as a disease marker. Tyr207 in CrkL is the BCR/Abl phosphorylation site (7). |
| 存放说明 | -20C |
| 计算分子量 | 39 |
Western blot analysis of extracts from K562 cells, untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-CrkL (Tyr207) Antibody (upper) or CrkL Antibody #3182 (lower).Western免疫印迹。用 Phospho-CrkL (Tyr207) Antibody (上图) 或 CrkL Antibody #3182 (下图)研究未经处理的和经小牛肠碱性磷酸酶(CIP)处理的K562细胞的细胞提取液。 | |
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, showing membrane and cytoplasmic localization, using Phospho-CrkL (Tyr207) Antibody.免疫组织化学染色分析石蜡包埋人肺癌组织。所用抗体为Phospho-CrkL (Tyr207) Antibody，图中所示为细胞膜和细胞质定位。 | |
Flow cytometric analysis of K562 cells, untreated (blue) or STI571 treated (green), using Phospho-CrkL (Tyr207) Antibody compared to a nonspecific negative control antibody (red).流式细胞仪分析K562细胞，未经处理(蓝色)和经过STI571 处理 (绿色) 所用抗体为Phospho-CrkL (Tyr207) 与非特异性对照抗体(红色)对比。 |
