| 货号 | 2561S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F/E-P |
| 使用方法 | WB(1:1000) IP (1:50) F (1:25) E-P (1:2000) |
| 供应商 | CST |
| 背景 | Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5).周期蛋白依赖性激酶2(p33CDK2)是细胞周期机制的重要组成部分。类似于p34cdc2，激酶活性的调节经由磷酸化状态以及周期蛋白亚基和CDK抑制剂之间的关系。磷酸化抑制通常发生在14位和15位苏氨酸(1)。CDK2-周期蛋白复合体的抑制也与p27 Kip1和 p21 Waf1/Cip1之间的关系有关(2)。CDK2复合体的磷酸化需要cdc25磷酸酶和160位苏氨酸磷酸化引(由CDK7和cyclin H组成的CAK复合体介导)起的14位和15位苏氨酸去磷酸化。CDK2/周期蛋白E激酶活性对于G1到S期的转换和Rb蛋白的磷酸化具有重要意义。在S期，活化的CDK2/周期蛋白A复合体支配和磷酸化E2F，而活化的CDK2复合体持续在G2期的胞核中存在(5)。 |
| 存放说明 | -20C |
| 计算分子量 | 33 |
Flow cytometric analysis of untreated Jurkat cells, using Phospho-CDK2 (Thr160) Antibody versus propidium iodide (DNA content). The boxed population indicates phospho-CDK2 (Thr160)-positive cells. 流式细胞术分析未处理的Jurkat细胞，使用的抗体为Phospho-CDK2 (Thr160) Antibody ，PI染色法检测DNA含量。框内的细胞群为phospho-CDK2 (Thr160)阳性细胞。 | |
Western blot analysis of extracts from HeLa cells synchronized in G0, G1/S or Mitosis, using Phospho-CDK2 (Thr160) Antibody. |
