| 货号 | 3901S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/F |
| 使用方法 | WB(1:1000) IHC-P (1:50) F (1:50) |
| 供应商 | CST |
| 背景 | The Bcr gene was orginally identified by its presence in the chimeric Bcr-Abl oncogene (1). The amino-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domains. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region may be involved in a GTPase activating function for the small GTP-binding protein Rac (2,3). The function of wild type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstream signaling mediator (4). The Bcr-Abl fusion results in production of a constitutively active tyrosine kinase, which causes chronic myelogenous leukemia (CML) (5). Tyr177 of Bcr is phosphorylated in the Bcr-Abl fusion protein, which plays an important role in transforming the activity of Bcr-Abl (6). Phosphorylated Tyr177 of Bcr provides a docking site for Gab2 and GRB2 (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 160 (Bcr); 210 (Bcr-Abl) |
Western blot analysis of extracts from K562 cells, untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-Bcr (Tyr177) Antibody (upper) or Bcr Antibody #3902 (lower).western blot方法检测细胞提取物：未经处理和牛小肠磷酸酶CIP)处理的 K562细胞，使用的抗体是Phospho-Bcr (Tyr177) Antibody (上图) 和 Bcr Antibody #3902 (下图)。 | |
Immunohistochemical analysis of paraffin-embedded bone marrow from a patient with chronic myelogenous leukemia, showing membrane and cytoplasmic localization, using Phospho-Bcr (Tyr177) Antibody.免疫组织化学方法检测石蜡包埋的白血病病人骨髓组织，显示胞膜和细胞质位置。使用的抗体是Phospho-Bcr (Tyr177) Antibody。 | |
Western blot analysis of extracts from PAE/CKR cells (expressing chimeric receptors of the extracellular domain of CSF-1R, and transmembrane and cytoplasmic domains of KDR) stimulated with CSF-1 (40 ng/ml for 5 minutes) or NIH/3T3 cells stimulated with PDGF (40 ng/ml for 2 minutes) using Phospho-Bcr (Tyr177) Antibody (upper) or Bcr Antibody #3902 (lower).western blot方法检测细胞提取物：CSF-1 (40 ng/ml，5 min)刺激的PAE/CKR细胞（表达CSF-1R胞外区域的嵌合受体）和NIH/3T3细胞刺激的PDGF (40 ng/ml，2 min)。使用的抗体是Phospho-Bcr (Tyr177) Antibody (上图) 和 Bcr Antibody #3902 (下图)。 | |
Flow cytometric analysis of K562 cells, untreated (green) or Gleevec® (STI571)- treated (blue), using Phospho-Bcr (Tyr177) Antibody compared to a nonspecific negative control antibody (red).Flow Cytometry方法检测细胞提取物：未经处理(绿色)的和Gleevec® (STI571)处理的（蓝色）K562细胞，使用的抗体是Phospho-Bcr (Tyr177)，红色表示非特异性阴性对照抗体。 |
