| 货号 | 9221S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IHC-F/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:250) IHC-P (1:50) F (1:50) IF-IC (1:50) IHC-F (1:50) |
| 供应商 | CST |
| 背景 | The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma showing nuclear localization, using Phospho-ATF-2 (Thr71) Antibody .免疫组织化学方法检测石蜡包埋的人乳腺癌组织，显示出细胞核的位置。使用的抗体是Phospho-ATF-2 (Thr71) Antibody | |
Immunohistchemical analysis of paraffin-embedded human colon carcinoma using Phospho-ATF-2 (Thr71) Antibody.免疫组织化学方法检测石蜡包埋的人结肠癌组织，使用的抗体是Phospho-ATF-2 (Thr71) Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-ATF-2 (Thr71) Antibody.免疫组织化学方法检测石蜡包埋的人肺癌组织，使用的抗体是Phospho-ATF-2 (Thr71) Antibody。 | |
Western blot analysis of extracts from HeLa, C6 and NIH/3T3 cells, untreated or anisomycin-treated, using Phospho-ATF-2 (Thr71) Antibody.Western blot 方法检测细胞提取物：未经处理的和茴香霉素处理的、HeLa、C6、NIH/3T3细胞，使用的抗体是 Phospho-ATF-2 (Thr71) Antibody. | |
Flow cytometric analysis of Jurkat cells, untreated (blue) or Anisomycin-treated (green), using Phospho-ATF-2 (Thr71) Antibody compared to a nonspecific negative control antibody (red).Flow Cytometry方法检测细胞提取物：未经处理(蓝色)的和茴香霉素处理的（绿色）、 Jurkat 细胞，使用的抗体是 Phospho-ATF-2 (Thr71) Antibody，红色表示非特异性阴性对照抗体。 | |
Immunhistochemical analysis of frozen H1650 xenograft using Phospho-ATF-2 (Thr71) Antibody.免疫组织化学方法检测石蜡包埋的冻存 H1650移植瘤组织，使用的抗体是Phospho-ATF-2 (Thr71) Antibody. | |
Confocal immunofluorescent analysis of HeLa cells, either untreated (left) or anisomycin-treated (right), using Phospho-ATF-2 (Thr71) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦荧光法检测：未经处理的（左侧）或茴香霉素处理的（右侧）HeLa 细胞，使用的抗体是Phospho-ATF-2 (Thr71) Antibody (绿色)，肌动蛋白纤维素已被 Alexa Fluor® 555 phalloidin (红色)标记。 |
