| 货号 | 8438S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb recognizes endogenous levels of caspase-7 protein only when cleaved at Asp198. |
| 使用方法 | WB(1:1000) F (1:1600) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processsing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspases-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine #9953 (1 μM, 3 hr; right), using Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HeLa细胞，未处理（左）或 Staurosporine #9953 （1 μM, 3 hr;右）处理，使用抗体为CleavedCaspase-7 (Asp198) (D6H1) Rabbit mAb 兔单抗（绿色）。DY-554 phalloidin（红色）标记肌动蛋白丝。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Western blot analysis of extracts from HeLa, C2C12, and H-4-II-E cells, untreated (-) or treated (+) with Staurosporine #9953 (1 μM, 3 hr), using Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb (upper) and Caspase-7 Antibody #9492 (lower). Western blot 分析 HeLa，C2C12 和 H-4-II-E 细胞提取物，未处理（-）和 Staurosporine #9953 （1 μM, 3 hr）（+）处理，使用抗体为CleavedCaspase-7 (Asp198) (D6H1) Rabbit mAb 兔单抗（上图）和 Caspase-7 Antibody #9492（下图）。 | |
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb. 流式细胞仪检测Jurkat细胞，未处理（蓝）和etoposide处理（绿），使用抗体为Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb 兔单抗。 |
