| 货号 | 5112S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 使用方法 | WB(1:1000) F (1:1600) |
| 供应商 | CST |
| 背景 | The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Western blot analysis of extracts from untreated or UV-treated COS cells, NIH/3T3 cells and C6 cells, using Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (upper), or ATF-2 (20F1) Rabbit mAb #9226 (lower).Western blot 方法检测细胞提取物：未经处理和紫外照射的COS、C6细胞，使用的抗体是Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (上图), or ATF-2 (20F1) Rabbit mAb #9226 （下图)。 | |
Flow cytometric analysis of THP-1 cells, untreated (blue) or Anisomycin treated (green), using Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb compared to a nonspecific negative control antibody (red).Flow cytometric方法检测细胞提取物：未经处理（蓝色）和茴香霉素处理的（绿色）THP-1 细胞，使用的抗体是Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb，红色表示非特异性阴性对照抗体。 |
