| 货号 | 5726S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IF-IC/F |
| 使用方法 | WB(1:1000) F (1:800) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059. |
| 存放说明 | -20C |
| 计算分子量 | 42, 44 |
Confocal immunofluorescent analysis of HeLa cells, treated with PDBu (100 nM, 15 min; left) or U0126 #9903 (10 μM, 2 hr; right), using Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G) Mouse mAb (green) and β-Actin (13E5) Rabbit mAb #4970 (red).使用PDBu (100 nM, 15 min; 左)或U0126 #9903 (10 μM, 2 hr; 右)处理后的HeLa细胞，使用Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G)Mouse mAb（绿色）和β-Actin (13E5)Rabbit mAb #4970（红色）进行激光共聚焦免疫荧光分析。 | |
Western blot analysis of extracts from various cell lines, serum-starved overnight and untreated (-) or treated with U0126 #9903, hEGF #8916, or TPA #4174 as indicated, using Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G) Mouse mAb (upper) or p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb #4696 (lower).血清饥饿过夜后未处理（-）或使用U0126 #9903, hEGF #8916,或TPA #4174处理的多种细胞系提取物，使用Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G)Mouse mAb（上）或p44/42 MAPK (Erk1/2) (L34F12)Mouse mAb #4696（下）进行western blot分析。 | |
Flow cytometric analysis of Jurkat cells, treated with U0126 #9903 (10 μM, 2 hr) (blue) or TPA #4174 (200 nM, 15 min) (green), using Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G) Mouse mAb. Anti-mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.经过U0126 #9903 (10 μM, 2 hr) (蓝色)或TPA #4174 (200 nM, 15 min) (绿色)的Jurkat细胞，使用Phospho-p44/42 MAPK (Erk1) (Tyr204)/(Erk2) (Tyr187) (D1H6G)Mouse mAb进行流式分析。Anti-mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4408作为二抗使用。 |
