| 货号 | 9106S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Mink/D.melanogaster/Zebrafish/Bovine/Pig |
| 来源宿主 | Mouse |
| 应用 | W/F |
| 使用方法 | WB(1:2000) F (1:2000) |
| 供应商 | CST |
| 背景 | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059. |
| 存放说明 | -20C |
| 计算分子量 | 42, 44 |
Western blot analysis of extracts from FGF treated SK-N-MC cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (upper) or control p44/42 MAPK (Erk1/2) Antibody #9102 (lower).western blot方法检测细胞提取物：FGF处理的K-N-MC细胞，使用的抗体是Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (u上图)和control p44/42 MAPK (Erk1/2) Antibody #9102 (下图)。 | |
Specificity and sensitivity of the Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb: This antibody reacts specifically with as little as 50 pg of phosphorylated MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated MAP kinase by Western blotting.Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb特异性敏感性分析：Western blotting分析发现该抗体能与仅仅50 pg的磷酸化MAP激酶发生特异性反应，但是不能与大于4 µg的非磷酸化MAP激酶发生反应。 | |
Flow cytometric analysis of Jurkat cells, untreated (red) or PMA-treated (blue) ; and untreated (green) or PMA-treated (purple) following 90-minute CIP treatment, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb.Flow cytometric方法检测细胞提取物：未经处理的(红色)、PMA处理的(蓝色)，以及未经处理的（绿色）、PMA（紫色）+90min CIP处理的Jurkat细胞，使用的抗体是Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb。 |
