| 货号 | 4856S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 and 236. |
| 使用方法 | WB(1:1000) F (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated (100 ng/ml, 20 min), using Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower). 使用Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb兔单抗 (上图)和S6 Ribosomal Protein (5G10) Rabbit mAb兔单抗 #2217 (下图)，免疫印迹（Western Blot）分析在NIH/3T3细胞系中磷酸化S6 核糖体蛋白(Ser235/236)和S6核糖体蛋白(5G10)蛋白水平，实验分为对照组和PDGF处理组(100 ng/ml, 20分钟)。 | |
Flow cytometric analysis of Jurkat cells, untreated (green), or LY294002, Wortmannin and U0126-treated (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb compared to a nonspecific negative control antibody (red). 与阴性非特异的对照抗体（红色）比较，使用Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb兔单抗，流式细胞术分析Jurkat细胞，细胞分为未处理组（绿色）和LY294002处理组（蓝色）。 | |
Confocal immunofluorescent images of HeLa cells serum-starved for 20 hrs (left), 20% serum-treated (center), or 20% serum-treated after preincubation with Rapamycin (FRAP/mTOR Inhibitor) #9904 and labeled with Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 使用Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb兔单抗（绿色）标记，共聚焦免疫荧光观察磷酸化S6 核糖体蛋白在HeLa细胞中的定位，细胞分别经血清饥饿培养20小时(左图)，20%血清处理(中图)或在Rapamycin (FRAP/mTOR Inhibitor) #9904孵育后加20%血清处理（右图）。DY-554 phalloidin标记微丝蛋白（红色）。蓝色伪彩 = DRAQ5™ (DNA 荧光染料). |
