| 货号 | 4514S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 使用方法 | WB(1:1000) F (1:400) |
| 供应商 | CST |
| 背景 | SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5). |
| 存放说明 | -20C |
| 计算分子量 | 44 |
Western blot analysis of extracts from various cell lines, untreated or treated with sorbitol or UV, as indicated, using Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb.western blot方法检测细胞提取物：未处理、根据说明用山梨醇处理的和紫外照射的各种细胞系。使用的抗体是Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb。 | |
Flow cytometric analysis of HT-29 cells, untreated (blue) or UV-treated (green), using Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb compared to a nonspecific negative control antibody (red).Flow Cytometry方法检测细胞提取物：未经处理(蓝色)的和紫外照射的（绿色）HT-29细胞，使用的抗体是Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb，红色表示非特异性阴性对照抗体。 |
