| 货号 | 5431S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 使用方法 | WB(1:1000) IP (1:200) F (1:200) |
| 供应商 | CST |
| 背景 | SHP-2 (PTPN11) is a ubiquitously expressed, nonreceptor protein tyrosine phosphatase (PTP). It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens, and extracellular matrices in the control of cell growth, differentiation, migration, and death (1). Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites (3). SHP-2 becomes phosphorylated at Tyr542 and Tyr580 in its carboxy-terminus in response to growth factor receptor activation (4). These phosphorylation events are thought to relieve basal inhibition and stimulate SHP-2 tyrosine phosphatase activity (5). Mutations in the corresponding gene result in a pair of clinically similar disorders (Noonan syndrome and LEOPARD syndrome) that may result from abnormal MAPK regulation (6). |
| 存放说明 | -20C |
| 计算分子量 | 72 |
Western blot analysis of extracts from C6 cells, serum-starved overnight and treated with PDGF (#9909, 50 ng/ml for 5 minutes), using Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb (upper) or total SHP-2 Antibody #3752 (lower).Western blot方法检测细胞提取物：PDGF((#9909, 50 ng/ml 5 min)处理的无血清培养过夜C6细胞。使用的抗体是Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb (上图)和 total SHP-2 Antibody #3752 (下图)。 | |
Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or PDGF-treated (green), using Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb.Flow cytometric方法检测细胞提取物：未处理的（蓝色）和λ磷酸酶PDGF处理的（绿色）NIH/3T3细胞，使用的抗体是Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb。 |
