| 货号 | 14745S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | SH2 domain-containing leukocyte protein of 76 kDa (SLP-76) is a hematopoietic adapter protein that is important in multiple biochemical signaling pathways and necessary for T cell development and activation (1). ZAP-70 phosphorylates SLP-76 and LAT as a result of TCR ligation. SLP-76 has amino-terminal tyrosine residues followed by a proline rich domain and a carboxy-terminal SH2 domain. Phosphorylation of Tyr113 and Tyr128 result in recruitment of the GEF Vav and the adapter protein Nck (2). TCR ligation also leads to phosphorylation of Tyr145, which mediates an association between SLP-76 and Itk, which is accomplished in part via the proline rich domain of SLP-76 and the SH3 domain of ITK (3). Furthermore, the proline rich domain of SLP-76 binds to the SH3 domains of Grb2-like adapter Gads (3,4). In resting cells, SLP-76 is predominantly in the cytosol. Upon TCR ligation, SLP-76 translocates to the plasma membrane and promotes the assembly of a multi-protein signaling complex that includes Vav, Nck, Itk and PLCγ1 (1). The expression of SLP-76 is tightly regulated; the protein is detected at very early stages of thymocyte development, increases as thymocyte maturation progresses, and is reduced as cells mature to CD4+ CD8+ double-positive thymocytes (5). |
| 存放说明 | -20C |
| 计算分子量 | 76 |
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with H2O2 (11 mM, 1 min; green), using Phospho-SLP-76 (Ser376) (D9D6E) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Flow cytometric analysis of human PBMCs, unstimulated (left) or stimulated (right) with anti-CD3 and anti-CD28 (both at 2 μg/ml, 1 hr, 37ºC), using Phospho-SLP-76 (Ser376) (D9D6E) Rabbit mAb and co-stained with a CD3 antibody. Stimulation increases phosphorylation of SLP-76 at (Ser376) in the CD3 (T cell) population (right plot, upper right quadrant) relative to unstimulated cells (left plot, upper right quadrant). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Western blot analysis of extracts from EL4 cells, untreated (-) or treated with H2O2 (11 mM, 1 min; +), using Phospho-SLP-76 (Ser376) (D9D6E) Rabbit mAb (upper) or SLP-76 Antibody #4958 (lower). | |
Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with H2O2 (11 mM, 1 min; +), using Phospho-SLP-76 (Ser376) (D9D6E) Rabbit mAb (upper) or SLP-76 Antibody #4958 (lower). |
