| 货号 | 9516S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb detects endogenous levels of Smad1/5 only when dually phosphorylated at Ser463 and Ser465. The antibody does not cross-react with other Smad-related proteins. |
| 使用方法 | WB(1:1000) F (1:400) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Flow cytometric analysis of HeLa cells, untreated (blue) or BMP-treated (green), using Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb compared to a nonspecific negative control antibody (red). 使用pho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb兔单抗对未处理（蓝色）或BMP处理（绿色）的HeLa细胞进行流式细胞分析，用非特异性抗体做阴性对照（红色）。 | |
Western blot analysis of extracts from untreated or BMP-4-treated HeLa or NIH/3T3 cells using Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb. 使用 Phospho-Smad1/5 (Ser463/465) (41D10)Rabbit mAb兔单抗对未处理或经过BMP-4处理的HeLa或NIH/3T3细胞提取物进行western blot分析。 | |
Confocal immunofluorescent analysis of HT-1080 cells, BMP-treated (left) and untreated (right), using Phospho-Smad5 (Ser463/Ser465) (41D10) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 使用Phospho-Smad5 (Ser463/Ser465) (41D10) Rabbit mAb兔单抗(绿色)对BMP-处理（左）和未处理（右）的HT-1080细胞进行激光共聚焦荧光分析。使用DY-554 phalloidin（红色）标记肌动蛋白丝。 | |
Confocal immunofluorescent analysis of HT1080 cells, serum-starved (left) or serum-starved then treated with hBMP2 (50 ng/ml, 30 min; right) using Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (green) and Cox IV (4D11-B3-E8) Mouse mAb #11967 (red). |
