| 货号 | 9211S |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogasterPig/S.cerevisiae |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). |
| 存放说明 | -20C |
| 计算分子量 | 43 |
Western blot analysis of extracts from UV-treated NIH/3T3 cells, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody (upper) or control p38 MAPK Antibody #9212 (lower).western blot方法检测细胞提取物：UV处理的NIH/3T3细胞，使用的抗体是Phospho-p38 MAPK (Thr180/Tyr182) Antibody (上图) 和control p38 MAPK Antibody #9212 (下图)。 | |
Flow cytometric analysis of Jurkat cells, untreated (green) or anisomycin treated (blue), using Phospho-p38 MAPK (Thr180/Tyr182) Antibody compared to a nonspecific negative control antibody (red).Flow cytometric方法检测细胞提取物：未经处理（蓝色）和茴香霉素处理的（绿色）Jurkat细胞，使用的抗体是Phospho-p38 MAPK (Thr180/Tyr182) Antibody，红色曲线表示非特异性阴性对照抗体。 | |
Western blot analysis of extracts from C6 cells, untreated or anisomycin-treated, and NIH/3T3 cells, untreated or UV-treated, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody (upper) or p38 MAPK Antibody #9212 (lower).western blot方法检测细胞提取物：未处理和茴香霉素处理的C6细胞、未处理和UV处理的NIH/3T3细胞，使用的抗体是Phospho-p38 MAPK (Thr180/Tyr182) Antibody (上图) 和p38 MAPK Antibody #9212 (下图)。 | |
Confocal immunofluorescent analysis of HeLa cells -/+ UV light, labeled with Phospho-p38 MAPK (green). Absence of staining in untreated cells (left) and nuclear localization in treated cells (right). Red = Actin filaments (phalloidin).激光共聚焦荧光法检测HeLa细胞，左侧未经处理的样品无染色，右侧经紫外照射的样品显示出细胞核的位置。肌动蛋白丝被Alexa Fluor® 555 鬼笔环肽标记，呈红色。 |
