Cleaved Caspase-3 (Asp175) Antibody【科瑞奥博】

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Cleaved Caspase-3 (Asp175) Antibody

货号： 9661S 基本售价： 3960.0 元规格： 100ul

产品信息

概述
货号	9661S
反应种属	Human/Mouse/Rat/Monkey
来源宿主	Rabbit
应用	W/IP/IHC-P/IHC-F/IF-IC/F
目标/特异性	Cleaved Caspase-3 (Asp175) Antibody detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases.
使用方法	WB(1:1000) IP (1:100) IHC-P (1:300) F (1:800) IF-IC (1:400) IHC-F (1:600)

性能
供应商	CST
背景	Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).
存放说明	-20C
计算分子量	17, 19

参考图片

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells untreated, staurosporine-treated (3hrs, 1 µM in vivo) or cytochrome c-treated (1hr, 0.25 mg/ml in vitro), using Caspase-3 Antibody #9662 (upper) or Cleaved Caspase-3 (Asp175) Antibody (lower).

Western blot 检测Hela、NIH/3T3 和 C6 细胞的细胞提取物，未处理和 staurosporine（星胞菌素）处理(3hrs, 1 µM，体内) 或cytochrome c（细胞色素C）处理 (1hr, 0.25 mg/ml，体外)，使用 Caspase-3 Antibody #9662 (上图) 和 Cleaved Caspase-3 (Asp175) Antibody (下图）。

Immunohistochemical analysis of paraffin-embedded human tonsil, showing cytoplasmic and perinuclear localization in apoptotic cells (low and high magnification), using Cleaved Caspase-3 (Asp175) Antibody.

免疫组化检测石蜡包埋的人扁桃体切片，显示凋亡细胞中的胞浆和核周定位（低倍和高倍），使用抗体为Cleaved Caspase-3 (Asp175) Antibody.

Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved Caspase-3 (Asp175) Antibody compared to a nonspecific negative control antibody (red).<

流式细胞术分析 Jurkat cells，未处理 (蓝色) or etoposide 处理 (绿色), 使用抗体为 Cleaved Caspase-3 (Asp175) Antibody，非特异性抗体阴性作为对照(红色).

Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) Antibody preincubated with control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide #1050 (right).

免疫组化检测石蜡包埋的小鼠胚胎，使用抗体为 Cleaved Caspase-3 (Asp175) Antibody，对照肽段 (左) or Cleaved Caspase-3 (Asp175) 阻断肽段 #1050 (右)孵育.

Immunohistochemical analysis using Cleaved caspase-3 (Asp175) antibody on SignalSlide^TM Cleaved Caspase-3 IHC controls #8104 (paraffin-embedded Jurkat cells, untreated (left) or etoposide-treated (right)).

免疫组化显示：使用抗体是 Cleaved caspase-3 (Asp175) antibody on SignalSlide^TM Cleaved Caspase-3 IHC controls #8104 (石蜡包埋 Jurkat cells, 未处理(左) or etoposide(依托泊苷)处理 (右)).

Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine #9953 treated (right), labeled with Cleaved Caspase-3 (Asp175) Antibody (green). Actin filaments have been labeled with Alexa Fluor^® 555 phalloidin (red). Blue pseudocolor = DRAQ5^® (fluorescent DNA dye).

共聚焦免疫荧光显示HT-29 cells 图片,未处理（左）或 Staurosporine (星胞菌素)处理（右）用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 标记#9953标记（绿色）。微丝用Alexa Fluor^® 555 phalloidin（鬼比环肽）（红色）。蓝色伪彩=DRAQ5^®（DNA荧光染料）。