| 货号 | 9255S |
| 反应种属 | Human/Mouse/Rat/Hamster/S.cerevisiae |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:2000) IP (1:250) F (1:400) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5). |
| 存放说明 | -20C |
| 计算分子量 | 46, 54 |
Western blot analysis of extracts from 293 cells, untreated or UV-treated (lanes 1 and 2), NIH/3T3 cells, untreated or UV-treated (lanes 3 and 4) and C6 cells, untreated or anisomycin-treated (lanes 5 and 6), using Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb.western blot方法检测细胞提取物：未处理和紫外照射的293 细胞（1、2号条带），未经处理的和紫外照射的COS细胞（3、4号条带），未处理和茴香霉素处理的C6细胞（5、6号条带）。使用的抗体是Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb。 | |
Flow cytometric analysis of Jurkat cells, untreated (green) or anisomycin-treated (blue), using Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb compared to a nonspecific negative control antibody (red).流式细胞方法检测未处理（绿色）和茴香霉素处理（蓝色）的Jurkat细胞。使用抗体为Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb，红色为非特异结合的阴性对照。 | |
Confocal immunofluorescent analysis of HeLa cells untreated (left) and anisomycin-treated (right) using Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (green). Actin filaments have been labeled with DY554 phalloidin (red).激光共聚焦荧光法检测HeLa细胞，左图为未处理的，右图为茴香霉素处理的，检测抗体为Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb ，呈绿色。肌动蛋白丝被DY-554鬼笔环肽标记，呈红色。 |
