| 货号 | 3165S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Zap-70 (D1C10E) XP® Rabbit mAb detects endogenous levels of total Zap-70. This antibody does not cross-react with other related proteins. |
| 使用方法 | WB(1:2000) IP (1:50) F (1:400) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Western blot analysis of extracts from Jurkat cells using Zap-70 (D1C10E) XP® Rabbit mAb.Western免疫印迹。用Zap-70 (D1C10E) XP® Rabbit mAb 研究Jurkat细胞的细胞提取液。 | |
Flow cytometric analysis of Ramos B cells (red) and Jurkat T cells (blue), using Zap-70 (D1C10E) XP® Rabbit mAb.流式细胞仪分析Ramos B (红色) 和Jurkat T (蓝色)细胞，所用的抗体为Zap-70 (D1C10E) XP® Rabbit mAb。 | |
Confocal immunofluorescent analysis of human tonsil using Zap-70 (D1C10E) XP® Rabbit mAb (red) showing colocalization with T-cells that have been labeled with CD3 Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光研究人源扁桃体组织。所用抗体为Zap-70 (D1C10E) XP® Rabbit mAb (红色)，图片显示了其与用CD3 Mouse mAb (绿色)标记的T细胞的共定位。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料 ) |
