| 货号 | 5033S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | ERp72 (D70D12) XP® Rabbit mAb detects endogenous levels of total ERp72 protein. |
| 使用方法 | WB(1:1000) F (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Secretory proteins translocate into the endoplasmic reticulum (ER) during synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess thioredoxin homology domains, including ER stress protein 72 (ERp72), constitute the PDI family (3,4). ERp72 contains three thioredoxin homology domains (3) and plays a role in the formation and isomerization of disulfide bonds (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 72 |
Confocal immunofluorescent analysis of PANC-1 cells using ERp72 (D70D12) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对PANC-1细胞使用ERp72 (D70D12) XP® Rabbit mAb兔单抗（绿色）和β-Actin (8H10D10) Mouse mAb #3700（红色）进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5®(荧光DNA染料)。 | |
Western blot analysis of extracts from various cell lines using ERp72 (D70D12) XP® Rabbit mAb. 对多种细胞系使用ERp72 (D70D12) XP® Rabbit mAb兔单抗进行Western blot分析。 | |
Flow cytometric analysis of Hep G2 cells using ERp72 (D70D12) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 使用ERp72 (D70D12) XP® Rabbit mAb兔单抗（蓝）和非特异的阴性对照抗体（红），对Hep G2细胞进行流式细胞术分析。 |
