| 货号 | 26632S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 使用方法 | WB(1:1000) F (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1) appears to be more highly expressed in the CNS as compared to other family members (12-14). Expression of GABARAPL1 is associated with prognosis of some cancers, including hepatocellular and breast cancer (15,16). Inhibition of GABARAPL1 expression in breast cancer cells attenuates autophagic flux, results in metabolic changes, and leads to cancer promoting activities (17). |
| 存放说明 | -20C |
| 计算分子量 | 14, 16 |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with chloroquine (50 μM, 24 hr; right), using GABARAPL1 (D5R9Y) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from HeLa, C2C12, and C6 cells, untreated (-) or treated with chloroquine (50 μM, overnight; +), using GABARAPL1 (D5R9Y) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human GABARAP protein (hGABARAP-Myc/DDK; +), human GABARAPL1 protein (hGABARAPL1-Myc/DDK; +), or human GABARAPL2 protein (hGABARAPL2-Myc/DDK; +) using GABARAPL1 (D5R9Y) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Flow cytometric analysis of HeLa cells using GABARAPL1 (D5R9Y) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
