GSK-3α (D80D1) XP® Rabbit mAb【科瑞奥博】

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GSK-3α (D80D1) XP® Rabbit mAb

货号： 4818S 基本售价： 3860.0 元规格： 100ul

产品信息

概述
货号	4818S
反应种属	Human/Mouse
来源宿主	Rabbit
应用	IF-IC/F
目标/特异性	GSK-3α (D80D1) XP® Rabbit mAb detects endogenous levels of total GSK-3α protein. The antibody does not cross-react with GSK-3β.
使用方法	F(1:100) IF-IC (1:100)

性能
供应商	CST
背景	Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). GSK-3α regulates the production of amyloid-β peptides, a major component of the plaques that accumulate with progression of Alzheimer disease. Administration of therapeutic concentrations of lithium, a GSK-3 inhibitor, attenuates amyloid-β production by specifically inhibiting the cleavage of amyloid precursor protein (APP) by γ-secretase, blocking accumulation of amyloid-β peptides in the brains of mice that overproduce APP (6).
存放说明	-20C
计算分子量	51

参考图片

Confocal immunofluorescent analysis of MEF/GSK-3 wildtype cells (left), MEF/GSK-3β (-/-) cells (center) and MEF/GSK-3α (-/-) cells (right), using GSK-3α (D80D1) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF/wildtype, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

免疫荧光共聚焦分析MEF/GSK-3野生型细胞（上）， MEF/GSK-3β缺陷型细胞（中）和MEF/GSK-3 α缺陷型细胞（下），使用的抗体是GSK-3α (D80D1) XP® Rabbit mAb兔单抗（绿色）。肌动蛋白纤维被DY-554 phalloidin标记（红色）。蓝色伪彩 = DRAQ5® #4084 (DNA荧光染料)。 (MEF/wildtype, GSK-3α (-/-) 和 GSK-3β (-/-) 细胞由University of Toronto的Jim Woodgett博士提供）。

Flow cytometric analysis of MEF/GSK-3β (-/-) cells (red), MEF/GSK-3α (-/-) cells (green), and MEF/GSK-3 wildtype cells (blue) using GSK-3α (D80D1) XP® Rabbit mAb. (MEF/GSK-3 wildtype, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

流式细胞术分析MEF/GSK-3β (-/-) 细胞（红色）， MEF/GSK-3 α (-/-) 细胞（绿色）和MEF/GSK-3野生型细胞（蓝色），使用的抗体是GSK-3α (D80D1) XP® Rabbit mAb兔单抗。 (MEF/wildtype, GSK-3α (-/-) 和GSK-3β (-/-) 细胞由University of Toronto的Jim Woodgett博士提供）。