| 货号 | 5545S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | hnRNP A0 (D8A3) XP® Rabbit mAb recognizes endogenous levels of total hnRNP A0 protein. This antibody does not cross-react with other hnRNP family members. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Heterogeneous nuclear ribonucleoprotein A0 (hnRNP A0) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). The A/B subfamily of hnRNP includes A1, A2/B1, A3, and A0. hnRNP A0 is phosphorylated at Ser84 by MAPKAPK-2 in response to LPS treatment in mouse macrophage cells, which might play a key role in stimulating translation of the TNF-α message (2). |
| 存放说明 | -20C |
| 计算分子量 | 32, 34 |
Western blot analysis of extracts from various cell lines using hnRNP A0 (D8A3) XP® Rabbit mAb. 使用hnRNP A0 (D8A3) XP® Rabbit mAb兔单抗，免疫印迹（Western Blotting）分析不同细胞系的提取物。 | |
Confocal immunofluorescent analysis of HeLa cells using hnRNP A0 (D8A3) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 共聚焦免疫荧光观察hnRNP A0蛋白在HeLa细胞定位，使用hnRNP A0 (D8A3) XP® Rabbit mAb兔单抗（绿色）标记。DY-554 phalloidin标记微丝蛋白（红色）。 | |
Flow cytometric analysis of HeLa cells using hnRNP A0 (D8A3) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 与非特异阴性对照抗体（红色）比较，使用hnRNP A0 (D8A3) XP®Rabbit mAb兔单抗 (蓝色)流式细胞术分析HeLa细胞。 | |
Western blot analysis of extracts from HeLa cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® hnRNP A0 siRNA I #6503 (+), using hnRNP A0 (D8A3) XP® Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower). 使用hnRNP A0 (D8A3) XP® Rabbit mAb兔单抗 (上图)和β-Actin (13E5) Rabbit mAb兔单抗 #4970 (下图)，免疫印迹（Western Blotting）分析HeLa细胞系提取物，细胞分别转染SignalSilence® Control siRNA (Unconjugated) #6568 (-) 和SignalSilence® hnRNP A0 siRNA I #6503 (+)。 |
