| 货号 | 9750S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | IGF-I Receptor β (D23H3) XP® Rabbit mAb detects endogenous levels of total IGF-I receptor β protein. This antibody does not cross-react with insulin receptor. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:400) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
Flow cytometric analysis of SK-UT-1 (blue) and MCF7 (green) cells using IGF-I Receptor β (D23H3) XP® Rabbit mAb. 对SK-UT-1（蓝）和MCF7（绿）细胞使用IGF-I Receptor β(D23H3) XP?兔单抗进行流式细胞仪分析。 | |
Confocal immunofluorescent analysis of MCF7 (left) and SK-UT-1 (right) cells using IGF-I Receptor β (D23H3) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对MCF7（左）和SK-UT-1（右）细胞使用IGF-I Receptorβ(D23H3)XP?兔单抗（绿）进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5?#4084(荧光DNA染料)。 | |
Western blot analysis of extracts from 293 (IGF-I receptor β+) and SK-UT-1 (IGF-I receptor β-) cells using IGF-I Receptor β (D23H3) XP® Rabbit mAb (upper) or β-Actin Antibody #4967 (lower). 对293 (IGF-I receptor β+)和SK-UT-1 (IGF-I receptor β-)细胞抽提液使用IGF-I Receptor β (D23H3) XP?兔单抗（上图）及β-Actin 抗体#4967（下图）进行Western blot分析。 |
