| 货号 | 3416S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | IKKε (D61F9) XP® Rabbit mAb detects endogenous levels of total IKKε protein. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation; Ser177 and Ser181 in the activation loop of IKKβ (serine 176 and 180 in IKKα) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from Raw 264.7 and KNRK cell lines using IKKε (D61F9) XP® Rabbit mAb.Western免疫印迹。用IKKε (D61F9) XP® Rabbit mAb研究Raw 264.7 和 KNRK 细胞系的细胞提取液。 | |
Confocal immunofluorescent analysis of RAW 264.7 (left) and C2C12 (right) cells using IKKepsilon (D61F9) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).共聚焦免疫荧光研究 RAW 264.7 (左图) 和 C2C12 (右图)细胞，所用抗体为 IKKepsilon (D61F9) XP® Rabbit mAb (绿色)。Blue pseudocolor = DRAQ5® (DNA荧光染料 ) | |
Flow cytometric analysis of Raw 264.7 cells using IKKε (D61F9) XP® Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
