Integrin α4 (D2E1) XP® Rabbit mAb【科瑞奥博】

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Integrin α4 (D2E1) XP® Rabbit mAb

货号： 8440S 基本售价： 3860.0 元规格： 100ul

产品信息

概述
货号	8440S
反应种属	Human/Mouse/Rat
来源宿主	Rabbit
应用	W/IP/IF-IC/F
目标/特异性	Integrin α4 (D2E1) XP® Rabbit mAb recognizes endogenous levels of total integrin α4 protein.
使用方法	WB(1:1000) IP (1:100) F (1:200) IF-IC (1:200)

性能
供应商	CST
背景	Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).   A pair of important α4 integrins, α4β1 and α4β7, interact with VCAM-1, fibronectin, and MAdCAM-1 at cell adhesions (3). Gene knockout and antibody blocking research reveal that α4 integrins play important roles in embryonic liver and heart development and in fetal lymphocyte homing (4-6). Phosphorylation at Ser988 within the cytoplasmic tail of integrin α4 blocks binding to paxillin and promotes leading edge migration (7,8).   On SDS-PAGE, integrin α4 can migrate at several different apparent molecular sizes, a 150 kDa mature protein and a 140 kDa precursor protein (a 180 kDa protein also exists under mild non-reducing conditions) (9). Integrin α4 has a cleavage site at Arg558, which results in a small portion of the protein as either an 80 kDa N-terminal or 70 kDa C-terminal fragment (10).
存放说明	-20C
计算分子量	70, 140, 150,

参考图片

Western blot analysis of extracts from various cell lines using Integrin α4 (D2E1) XP® Rabbit mAb.Western blot分析多种细胞系提取物，使用的抗体是Integrin α4 (D2E1) XP® Rabbit mAb。

Confocal immunofluorescent analysis of Jurkat (positive; left) and K-562 (negative; right) cells using Integrin α4 (D2E1) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光分析Jurkat (阳性，左图)和K-562 (阴性，右图)，使用的抗体是Integrin α4 (D2E1) XP® Rabbit mAb (绿色)，蓝色伪彩 = DRAQ5® #4084 (DNA荧光染料)。

Flow cytometric analysis of Jurkat cells using Integrin α4 (D2E1) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.流式细胞术分析Jurkat细胞，使用的抗体是 Integrin α4 (D2E1) XP® Rabbit mAb (蓝色) ，以及与之匹配的相同浓度的 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (红色). 二抗为Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412。