| 货号 | 8641S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 使用方法 | WB(1:1000) F (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
Confocal immunofluorescent analysis of NTERA-2 cl.D1 (left) and HeLa (right) cells using Lin28A (D1A1A) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
使用Lin28A (D1A1A) XP® Rabbit mAb兔单抗 (绿色)对NTERA-2 cl.D1 (左) 和HeLa细胞 (右)进行共聚焦免疫荧光分析。肌蛋白丝用DY-554 phalloidin (红色) 标记。蓝色伪彩＝DRAQ5® #4084 (DNA萤光染料). |
