| 货号 | 4380S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 使用方法 | WB(1:1000) F (1:800) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. |
| 存放说明 | -20C |
| 计算分子量 | 120, 300 |
Western blot analysis of extracts from HBP-ALL, and Molt4 using Notch1 (D6F11) XP® Rabbit Ab. The full-length (FL) and transmembrane/intracellular region (NTM) are indicated. 使用Notch1 (D6F11) XP® Rabbit Ab对HBP-ALL和Molt4提取物进行western blot分析。Notch1 全长蛋白（FL）和跨膜/细胞内区域（NTM）如图所示。 | |
Flow cytometric analysis of Jurkat cells using Notch1 (D6F11) XP® Rabbit Ab (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 使用Notch1 (D6F11) XP® Rabbit Ab （蓝色）与相应浓度的Rabbit (DA1E) mAb IgG XP®同型对照 #3900 （红色）对Jurkat细胞进行流式细胞分析。 | |
Confocal immunofluorescent analysis of MCF7 cells using Notch1 (D6F11) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Notch1 (D6F11) XP® Rabbit mAb (绿色)对MCF7细胞进行激光共聚焦荧光分析。蓝色伪彩=DRAQ5® #4084 (DNA荧光染料). |
